Antitumor properties of an anti-idiotypic monoclonal antibody in relation to N-glycolyl-containing gangliosides

VÁZQUEZ, ANA MARÍA; GABRI, MARIANO R.; HERNÁNDEZ, ANA MARÍA; ALONSO, DANIEL F.; BEAUSOLEIL, IRENE; GOMEZ, DANIEL E.; PÉREZ, ROLANDO

ONCOLOGY REPORTS

SPANDIDOS PUBL LTD

Año: 2000 vol. 7 p. 751 - 756

1021-335X

We examined the antitumor effects of 1E10 monoclonal antibody, an anti-idiotypic IgG to an IgM monoclonal antibody, named P3, that reacts specifically with N-glycolyl-containing gangliosides and also recognizes antigens in human breast and melanoma tumors. Two murine tumor cell lines positive for the P3 antibody, F3II mammary carcinoma (BALB/c) and B16 melanoma (C57BL/6), were employed. In BALB/c mice, vaccination with several i.p. doses at 14-day intervals of 50 μg of 1E10 coupled to keyhole limpet hemocyanin in Freund´s adjuvant, significantly reduced s.c. tumor growth of F3II carcinoma cells and the number of spontaneous lung metastases. Also, the effect of 1E10 as a biological response modifier on tumor lung colonization was evaluated in C57BL/6 mice injected i.v. with B16 melanoma cells. Interestingly, i.v. administration of 10 μg of uncoupled 1E10 antibody, 10-14 days after inoculation of B16 cells, dramatically reduced the number of experimental metastases in comparison with lungs from mice treated with an irrelevant IgG. The present data suggest that this ´non-internal image´ anti-idiotypic monoclonal antibody may activate more than one mechanism of antitumor response against melanoma and mammary tumor cells.