INVESTIGADORES
MADRID Rossana Elena
congresos y reuniones científicas
Título:
IMPEDANCE SPECTROSCOPY MEASUREMENTS OF A LABEL FREE FUNCTIONALIZED SILICON SENSOR
Autor/es:
RICARDO M TRUJILLO; ANNA CATTANI-SCHOLZ; ACHYUT BORA; VEDRAN VANDALO; ROSSANA MADRID; MARC TORNOW
Lugar:
Mar del Plata
Reunión:
Congreso; XVIII Congreso Argentino de Bioingeniería y VII Jornadas de Ingeniería Clínica, SABI 2011 junto con 2º Taller de Órganos Artificiales, Biomateriales e Ingeniería de Tejidos, OBI 2011; 2011
Institución organizadora:
Soc. Arg. de Bioingeniería
Resumen:
There is not current commercial methods that allows to measure in real time biological events or quantify molecules. The main disadvantage of present methods is that samples must be labeled before the measurements. This work describes a possibility of label-free biosensing based on crystalline silicon functionalized with organic molecules. In order to get a response to changes on the sensor´s surface a special oxidation technique is needed. This technique allows to obtain uncontaminated ultra-thin layers and a very good quality of self-assembled monolayers (SAMs). Electrochemical impedance spectroscopy was used to analyze two particular cases of electrode functionalization: 1)protein bonding and 2) non-specific adsorption of λ-DNA over a self-assembled monolayer of molecules with different ending groups.