In Vitro Assessment of Chitosan Membranes as Wound Dressing for Skin Regeneration

ANA GONZALEZ WUSENER; IGNACIO RUIZ; ALBERTO BOLGIANI; CARLOS ARREGUI; ELIDA HERMIDA

Boston

Conferencia; World Conference of the Tissue Engineering and Regenerative Medicine International Society, TERMIS 2015,; 2015

TERMIS

In Vitro Assessment of Chitosan Membranes as Wound Dressing for Skin RegenerationAna González Wusener1,2, Ignacio E. Ruiz2,3, Alberto N. Bolgiani4, Carlos Arregui1,2, Élida B. Hermida2,31.Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH); UNSAM-CONICET; Buenos Aires, Argentina2.Consejo Nacional de Investigaciones Científicas y Técnicas; Buenos Aires, Argentina.3.Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Buenos Aires, Argentina.4.Centro de Excelencia para Asistencia de Quemaduras (CEPAQ) Fundación Benaim, Hospital Alemán, Buenos Aires, Argentina.Chitosan is a linear copolymer of β-(1-4) linked 2-acetamido-2-deoxy-β-D-glucopyranose and 2-amino-2-deoxy-β-D-glucopyranose. It is obtained through the N-deacetylation of chitin, a readily available structural polysaccharide present in invertebrate exoskeleton as well as fungi cell wall.Chitosan is biocompatible, biodegradable and the degradation products are resorbable. Moreover, this biomaterial has antimicrobial activity and low Immunogenicity. It stimulates cell proliferation and tissue organization and it is a hemostat, helping in natural blood clotting.Due to these properties, chitosan is a promising material for skin regeneration therapies. This contribution presents in vitro studies performed with neutral chitosan membranes developed and optimized in our laboratory. Cytotoxicity and cell adhesion and proliferation studies were performed using murine and human cells. Results of these in vitro tests allow claiming that chitosan scaffolds show excellent potential in the management of wounds and burns.Acknowledgements: This work has been partially supported by FONARSEC, Project EMPRETECNO N° 37, the National University of San Martín (UNSAM) and the National Council of Scientific and Technical Research (CONICET).Disclosures: The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.