Evaluation of culture medium on poly(3-hydroxybutyrate) production by Cupriavidus necator ATCC 17697: application of the response surface methodology

DAIANA NYGAARD; OXANA YASHCHUK; ELIDA B HERMIDA

HELIYON

Elsevier

Año: 2019 vol. 5 p. 1 - 18

2405-8440

Polyhydroxyalkanoates (PHA), of which polyhydroxybutyrate (PHB) is the mostabundant, are polymers of bacterial origin used for various applications in themedical, industrial and agricultural fields. In the present study we worked on the selection, evaluation and improvement of the significant variables of the medium for the production of PHB by Cupriavidus necator ATCC 17697. In order toaddress the selection of the main factors and optimize the culture medium, acomplete factorial experimental design based on the coupled response surfacemethodology, was presented. The model with the best adjustment of thevariables turned out to be quadratic in fructose (C), linear in ammonium sulphate (N) and pH, with interaction in pH and phosphate solution (P), where the pH was the most significant (p < 0.0001) while the micro-elements solution could be neglected. Thus, optimum carbon concentration, adequate nitrogen limitation and interaction between initial pH and phosphate solution concentration are important factors to ensure a high production of PHB. The optimal values of the selected variables were C ¼ 20 g/l, N ¼ 1.5 g/l, P ¼ 8.75 g/l and pH 7.5. A maximum PHB production of 4.6 g/l, obtained under these conditions, increased almost 2.5 times. The polymer accumulated in the cytoplasm of C. necator ATCC 17697 in the form of granules showed an FTIR spectrum corresponding to that of commercial PHB.