INVESTIGADORES
GIAMBARTOLOMEI Guillermo Hernan
congresos y reuniones científicas
Título:
Type 4 secretion system (T4SS) and the secreted protein BPE005 from Brucella abortus modulates hepatic stellate cells responses.
Autor/es:
ARRIOLA BENITEZ P. C., PESCE VIGLIETTI A. I., REY SERANTES D., HERRMANN C. K., VANZULLI S., COMERCI D. J. GIAMBARTOLOMEI G. H., DELPINO M. V.
Reunión:
Congreso; LXIII Reunión Anual de la Sociedad Argentina de Inmunología.; 2015
Resumen:
The liver isaffected in human brucellosis. Hepatic stellate cells (HSC) are the major cellsinvolved in liver fibrosis, and they are the resident antigen-presenting cells.Brucella abortus (Ba) could beinvolved in the modulation of immune response in liver through activation ofinflammasome with concomitant fibrosis to repair the damage. Ba tilts HSC to a profibrogenic phenotype in a way that involves afunctional T4SS and the secreted protein BPE005. Todetermine in vivo relevance of the role of BPE005 in liver fibrosis, BALB/c mice were infected with Ba and bpe005 mutant. Masson's trichrome(p<0.05) and Sirius red (p<0.01) staining revealed that the presence of fibrotic patches was lower in miceinfected with Ba bpe005 mutant than in those infected with Ba.TGF-β1 secretion was lower in livers from Ba bpe005mutant-infected mice than those infected with Ba (p<0.05), and HSC are the main cells involved in TGF-β1secretion as was revealed by immunohistochemistry. HSC could expressinflammasomes, our results using LX-2 cell line indicated that Ba inducethe secretion of IL-1β in a mechanism dependent on the presence of a functionalT4SS but not the BPE005 protein (p<0.001). Experiments conducted todetermine if Ba could modulate LX-2 cells antigen presenting cells,revealed that Ba infection induced MHC-I (p<0.01) and MHC-II (p<0.001)expression, but not affect the expression of (CD40, CD80 and CD86) in LX-2. These results indicate that the T4SS and BPE005 are involved in most of themechanisms involved in the modulation of liver responses.
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