Role of a Ca2+-activated K+ current in the maintenance of resting membrane potential of isolated human saphenous vein smooth muscle cells

MILESI, MARIA VERÓNICA; AIELLO, ALEJANDRO; REBOLLEDO, ALEJANDRO; GOMEZ ALVIS, ALICIA; GRASSI DE GENDE AO

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY

SPRINGER

Lugar: Berlin; Año: 1999 p. 455 - 461

0031-6768

Calcium-activated potassium currents werestudied in dissociated smooth muscle cells from humansaphenous vein (HSV) using the patch-clamp techniquein the whole-cell configuration. The average measuredresting membrane potential (Vm) was ?41±2 mV (n=39),when the cells were dialysed with an intracellular pipettesolution (IPS) containing 0.1 mM ethyleneglycolbis(b-aminoethylether)-N,N,N¢,N¢-tetraacetic acid (EGTA)(IPS-0.1 mM EGTA). When the EGTA concentration wasincreased to 10 mM (IPS-10 mM EGTA) Vm becamesignificantly less negative: ?13±2 mV (n=23, P