ENDOTHELIUM-DEPENDENT RESPONSE TO ANGIOTENSIN II AND NORADRENALINE IN RABBITS FEED ON A HIGH FAT DIET

SCACHI FABRICIO; SIERRA L; GUERRERO R.; PERAL DE BRUNO MARIA.; JEREZ S.

Rosario

Congreso; LXI Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE); 2009

ociedad Argentina de Farmacología Experimental (SAFE)

ENDOTHELIUM-DEPENDENT RESPONSE TO ANGIOTENSIN II AND NORADRENALINE IN RABBITS FEED ON A HIGH FAT DIET Scacchi F, Sierra L., Guerrero R, Peral M. and Jerez S. INSIBIO (UNT-CONICET). fabrizioscacchi@yahoo.com.ar The aim of this work was to study the role of endothelium in the contractile response to angiotensin II (Ang II) and noradrenaline (NA) in rabbits feed on a high fat diet. Rabbits were feed with either normal rabbit chow (CD) or a high fat diet containing 6,67% corn oil and 3,33% lard (FD) ad libitum for 12 weeks. Thoracic aorta was excised, rings were cut and mounted in an organ bath to register isometric contractions in endothelium intact (E+) or endothelium removed (E-) arteries. One cumulative dose response curves (CDRC) to acetylcholine (Ach) was performed. After washing, one CDRC to NA or AngII was performed in E(+) and E(-) arteries. In other groups, E(+) arteries were incubated with indomethacin 10-5 M or miconazol 10-6 M or L-NAME 10-4 M before CDRC to NA or Ang II. Results: affinity to Ach was lower (pD2: 6.82±0.14 vs 7.08±0.09, p