“Switch in placental T cell populations after dendritic cell therapy in a CBA/J x DBA/2 abortion prone mating” (II)

MIRANDA S, LITWIN S, BARRIENTOS G, SZEKERES-BARTHO J AND BLOIS S

Córdoba, Argentina.

Congreso; VII Congreso Latinoamericano de Inmunología.; 2005

Asociación Latinoamericana de Inmunología (ALAI) en conjunto con la Sociedad Argentina de Inmunología (SAI), la Sociedad Chilena de Inmunología (SOCHIN), la Sociedad Latinoamericana de Histocompatibilidad (SLAH) y el Grupo de Trabajo de Inmunología Pediát

Switch on Placental T-Cell Populations after a Dendritic Cell Therapy in the CBA/J x DBA/2J Abortion-prone Mouse Model S. Miranda1, S. Litwin1, G. Barrientos1, J. Szekeres-Bartho2 and S. Blois3 1 IDEHU (CONICET-UBA), Argentina, 2 University of Pécs, Hungary, 3 Charite, Medicine University of Berlin, Germany. Aim of Study: Therapy with syngeneic dendritic cell (DC) proved to diminish the resorption rate (%R) in the CBA/J x DBA/2J crossbreeding. The underlying mechanisms remain unknown. The presence of TCRγδ, CD8α, PIBF and PIBF-REC+ cells in maternal-fetal interface after such therapy is now investigated. Material and Methods: CBA/J females were inoculated with syngeneic bone-marrow derived DC twice before mating. The studied groups were: 1- control, 2- mice injected with DC culture medium (IMDM-GM-CSF), 3-immunized with DC alone and 4- immunized with paternal DBA/2J antigens lisate-pulsed DC, n=5. The presence of TCRγδ, CD8α, PIBF and PIBF-REC+ cell in placental tissue was analyzed by immunohistochemistry.  Depending on the number of positive cells, the data were scored to allow statistical analysis. Results:.The table shows the statistical differences of the results respect to both controls. Treatment % R CD8a TCR gd PIBF R-PIBF Control 23.8 = = = = IMDM-GM-CSF 17.6 = = = = Antigen-pulsed DC 5.0 ­** ­* = =  DC alone 2.2 ­­*** ­­** ­* mild ¯ Discussion: CD therapy induced an increase in placental TCRγδ+, CD8α+, PIBF+ cells, specially employing DC alone. We suggest that CD therapy up-regulate a protective/regulatory population of T-cell in maternal-fetal interface which would be responsible for the corrected %R obtained.