INVESTIGADORES
MIRANDA Silvia Esther
congresos y reuniones científicas
Título:
¡°Switch in placental T cell populations after dendritic cell therapy in a CBA/J x DBA/2 abortion prone mating¡± (I)
Autor/es:
MIRANDA S, LITWIN S, BARRIENTOS G, SZEKERES-BARTHO J, ARCK P AND BLOIS S
Lugar:
Essex, United Kingdom
Reunión:
Congreso; Third European Congress of Reproductive Immunology; 2005
Institución organizadora:
The Reproductive Immunology Group (RIG) of the British Society for Immunology
Resumen:
Switch on T-Cell Populations after Dendritic Cell Therapy in THE CBA/J x DBA/2J Abortion-prone Mouse Model S. Miranda1, S. Litwin1, G. Barrientos1, J. Szekeres-Bartho2, PC Arck3, S. Blois3 1 IDEHU (CONICET-UBA), Argentina, 2 University of P¨¦cs, Hungary, 3 Medicine University of Berlin, Germany. Third European Congress of Reproductive Immunology. Essex, UK. 2005      Introduction: Therapy with syngeneic dendritic cell (DC) diminished the resorption rate (%R) in the CBA/J x DBA/2J crossbreeding. The underlying mechanisms remain unknown. The presence of TCR¦Ã¦Ä, CD8¦Á, PIBF and PIBF-Receptor (REC)+ cells after such therapy is now investigated. Material and Methods: CBA/J females were inoculated with syngeneic bone-marrow derived DC twice before mating. The studied groups were: 1- no treatment control, 2- mice injected with DC culture medium (DCCM), 3-immunized with DC alone and 4- immunized with paternal DBA/2J antigens pulsed DC, n=5. The presence of TCR¦Ã¦Ä, CD8¦Á, PIBF and PIBF-REC+ cell in placental tissue was analyzed by immunohistochemistry. Depending on the number of positive cells, the data were scored to allow statistical analysis. Results: DC therapy induced an increase in the number of CD8¦Á and TCR¦Ã¦Ä positive cells, especially with the therapy employing DC alone. Non-treated mice showed no significant differences in the expression of both CD8¦Á and TCR¦Ã¦Ä respect to the animals inoculated with DCCM, while PIBF expression was increased only in the syngeneic DC alone treatment group. DC therapy did not appear to influence the expression of PIBF-REC. Conclusions: We suggest that DC therapy up-regulate a protective population TCR¦Ã¦Ä+, CD8¦Á+, PIBF+ in maternal-fetal interface which would be responsible for the corrected %R obtained.