Multiparity increases VEGF in maternal-fetal interface. Subnormal behaviour of the CBA/J x DBA/2 crossbreeding?

BARRIENTOS G; LITWIN S; MIRANDA S

Santiago de Chile

Simposio; II Simposio Latinoamericano de Interacción materno-fetal & Placenta.; 2005

Sociedad Latinoamericana de Interacción materno-fetal & Placenta. Santiago de Chile.

multiparity increases VEGF in maternal-fetal interface. SUBNORMAL BEHAVIOUR OF THE CBA/J X DBA/2 CROSSBREEDING aG. Barrientos, aS. Litwin, bE. Roux, aS. Miranda aIDEHU(CONICET-UBA); bCát. de Fisiopatología. FFyB-UBA. Junín 956 4P (1113). Bs As, Argentina. II Simposio Latinoamericano de Interacción materno-fetal & Placenta. Santiago de Chile. 2005. We previously reported that placentae from multiparous females showed an increased invasive trophoblast tissue. The aim of this work was to study the influence of multiparity on the presence of VEGF, VEGF-R1 and sVEGF-R1 in placenta and serum. Methods: CBA/J x CBA/J, CBA/J x BALB/c and the abortion-prone CBA/J x DBA/2 mouse combinations were divided into three groups: Primiparous Young (PY): 3.0 ± 0.5 months old, n =10; Primiparous Old (PO): 8.5 ± 0.5 months old, n =10 and Multiparous Old (MO): 8.5 ± 0.5 months old with 4 pregnancies, n =10. Sera and placentae were obtained at term pregnancy. Placental VEGF expression was investigated by IHC. VEGF-R1 levels in PS and sera were measured by ELISA. Results: All groups showed similar quantity of VEGF+ cells in the whole trophoblast. Among the PY groups, CBA/J x DBA/2 presented low VEGF+ cells in the area next to decidua (p<0.01). In this zone, all MO combinations showed higher expression of VEGF (p< 0.001). In non- pregnant CBA/J mouse sera VEGF-R1 level was 559 ± 172 pg/ml but at term of the first pregnancy, the CBA/J x Balb/c group showed a high increase  (PY: 5900 ± 532 / PO: 8062 ± 1221) respect  to CBA/J x DBA/2 sera (PY: 3003 ± 553 / PO: 3134 ± 238). Sera from MO mice showed lower levels (CBA/J x Balb/c: 2212 ± 360 and CBA/J x DBA/2: 1015 ± 230). PS did not show significant differences. Conclusions: Multiparity status induces an increase of VEGF expression by invasive trophoblast. The CBA/J x DBA/2 combination showed a lower effect. Our data suggest that VEGF could be involved in the invasion process. The diminished serum VEGF-R1 level found in MO females would also increase VEGF concentration. The biological significance of this systemic effect has to be further investigated. Supported by UBA (B097).