INVESTIGADORES
MIRANDA Silvia Esther
congresos y reuniones científicas
Título:
Switch in placental T cell populations after dendritic cell therapy in a CBA/J x DBA/2 abortion prone mating (II)
Autor/es:
MIRANDA S; LITWIN S; BARRIENTOS G; BLOIS S
Lugar:
Córdoba
Reunión:
Congreso; VII Congreso Latinoamericano de Inmunología.; 2005
Institución organizadora:
Asociación Latinoamericana de Inmunología (ALAI) en conjunto con la Sociedad Argentina de Inmunología (SAI), la Sociedad Chilena de Inmunología (SOCHIN), la Sociedad Latinoamericana de Histocompatibilidad (SLAH) y el Grupo de Trabajo de Inmunología Pediát
Resumen:
Switch on Placental T-Cell Populations after a Dendritic Cell Therapy in the CBA/J x DBA/2J Abortion-prone Mouse Model
S. Miranda1, S. Litwin1, G. Barrientos1, J. Szekeres-Bartho2 and S. Blois3
1 IDEHU (CONICET-UBA), Argentina, 2 University of Pécs, Hungary, 3 Charite, Medicine University of Berlin, Germany.
Aim of Study: Therapy with syngeneic dendritic cell (DC) proved to diminish the resorption rate (%R) in the CBA/J x DBA/2J crossbreeding. The underlying mechanisms remain unknown. The presence of TCRγδ, CD8α, PIBF and PIBF-REC+ cells in maternal-fetal interface after such therapy is now investigated.
Material and Methods: CBA/J females were inoculated with syngeneic bone-marrow derived DC twice before mating. The studied groups were: 1- control, 2- mice injected with DC culture medium (IMDM-GM-CSF), 3-immunized with DC alone and 4- immunized with paternal DBA/2J antigens lisate-pulsed DC, n=5. The presence of TCRγδ, CD8α, PIBF and PIBF-REC+ cell in placental tissue was analyzed by immunohistochemistry. Depending on the number of positive cells, the data were scored to allow statistical analysis.
Results:.The table shows the statistical differences of the results respect to both controls.
Treatment
% R
CD8a
TCR gd
PIBF
R-PIBF
Control
23.8
=
=
=
=
IMDM-GM-CSF
17.6
=
=
=
=
Antigen-pulsed DC
5.0
**
*
=
=
DC alone
2.2
***
**
*
mild ¯
Discussion:
CD therapy induced an increase in placental TCRγδ+, CD8α+, PIBF+ cells, specially employing DC alone. We suggest that CD therapy up-regulate a protective/regulatory population of T-cell in maternal-fetal interface which would be responsible for the corrected %R obtained.