Acoustic stress induces long term severe intestinal inflammation in the mouse

MIRANDA S; ROUX ME

TOXICOLOGY LETTERS

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2017

0378-4274

The influence of noise on the presentation and progression of inflammatory bowel diseases has been poorly analyzed. We designed this study to investigate immediate and late effects of acoustic stress (AS) on small intestine. To this aim, CBA/J, BALB/c and DBA/2 mice were divided into AS and control groups. AS mice were exposed to noise (300Hz-70dB) during 24hs and randomized into: A) Acute effects group: mice were killed after AS; L) Late effects group: mice were killed 3 weeks after AS and O) Over-exposed effects group: mice were submitted to AS once a week during a month and killed. Small intestine sections were histologically examined. The expression of cytokines (IL-17, IL-22, TNF-α, INF-ɣ and TGF-β), CCL-25 and Ki67 was studied by immunohistochemistry and immunofluorescence techniques. ?A? group displayed short and fragmented villi, diminished number of lamina propria cells, leucocyte infiltration, higher number of goblet cells and predominance of IL-17 expression. ?L? group showed epithelial proliferative foci (CCL25+Ki67+) and increased TNFα/TGF-β expression. Tissue damage was aggravated in ?O? group. In conclusion, AS is able to trigger a severe intestinal inflammatory process in healthy mice, which spontaneously amplifies and perpetuates. Noise might be harmful to humans by aggravating inflammatory bowel diseases.