OZONETHERAPY PROTECTS AGAINST CORONARY RESTENOSIS REDUCING NEOINTIMAL HYPERPLASIA AND INFLAMMATION. ROLE OF TRX-1

BARONE A.; OTERO-LOSADA M.; CAO G; AZZATO F.; RODRIGUEZ A.; MILEI J.

Paris

Congreso; European Society of Hypertension ESH 2016 Annual Meeting; 2016

European Society of Hypertension

Objective. To evaluate whether ozonotherapy might reduce restenosis following bare metal stents (BMS) implantation related to antioxidant enzymes.Design and Method. Twelve male Landrace pigs (51±9 kg) underwent percutaneous transluminal circumflex coronary arteries (CCA) BMS implantation, fluoroscopically guided, under heparine infusion (100 UI/kg). Pigs were randomized to ozonetherapy (n=6) or placebo (n=6). Before stenting (24 h) and twice a week for 30 days post-stenting, venous blood (100 mL) was collected, bubbled with an ozone-oxygen mixture to 20 µg/mL ozone final concentration, and reinfused through the jugular vein. Same procedure was performed in placebo group except for ozonation. Both groups received aspirin 300 mg/day and clopidogrel 300 mg (24 h pre-stenting + 75 mg/day during follow-up). Arterial tree was monitored (cinecoronariography). Coronary flow was categorized (TIMI score). Thirty days after implantation, pigs were euthanized. CCA were excised and processed for histomorphometry (H&E, Masson trichrome) and immunohistochemistry [antibodies against CD68 (monocytes/macrophages), CD34 (endotelial cells), alpha-actin (SMC), thioredoxin (Trx)-1 and peroxiredoxin (Prx)-2]. Between-treatment comparison: unpaired ?t? and Mann-Whitney tests, statistical significance p