INVESTIGADORES
OTERO-LOSADA Matilde Estela
congresos y reuniones científicas
Título:
Pleiotropic effects of antihypertensives in genetically hypertensive rats
Autor/es:
GOMEZ-LLAMBI H.; OTTAVIANO G; CAO G; MULLER A; OTERO-LOSADA M.; MILEI J.
Lugar:
Rome
Reunión:
Congreso; European Society of Cardiology. Annual Congress; 2016
Institución organizadora:
European Society of Cardiology
Resumen:
Introduction. Elsewhere in this meeting, we report changes in cardiac function in spontaneously hypertensive rats (SHRs) treated with different antihypertensive drugs.Purpose. To evaluate whether drugs usually used as antihypertensives, might affect cardiac morphology in experimental hypertension.Methods. Two months old SHRs were assigned to different groups (n = 18/group) and treated (mg/kg, p.o) with: losartan 30 (L), hydralazine 11 (H), rosuvastatin 10 (R), carvedilol 20 (C) or water (SHRw control treatment). A control group for hypertension comprising 18 normotensive rats (Wistar-Kyoto, WKY) was also studied. Systolic blood pressure (SBP) (tail plethysmography in awake animals) and body weight (BW) were measured periodically. Euthanasia was administered at 16 months of treatment. The hearts were immediately excised and conventionally processed for morphology/morphometry (light microscopy). Images were digitalized and analyzed (Image-Pro Plus 4.5). Morphometry. Volume density of: Vv myocardium (Vv myo), Vv interstitium (Vv int) and Vv coronary microvessels (Vv cmv) was estimated and expressed as percentage. Individual myocyte mean cross-sectional area (MCSA, µm2) was determined and the number of myocytes per mm2 was estimated. Coronary microvessels? density (CMD) was expressed as microvessels/mm2 cross-sectional area of myocardium. Diffusion distance was calculated as R = 103/√Nπ, (N=number of capillaries/ mm2).Results. Findings in LV. Compared with SHRw, interstitial volume (%) decreased in groups L and H (8±1, p