PLEIOTROPIC EFFECTS OF ANTIHYPERTENSIVE PHARMACOLOGICAL TREATMENTS IN SPONTANEOUSLY HYPERTENSIVE RATS

GOMEZ-LLAMBI H; CAO G; OTTAVIANO G; MULLER A; OTERO-LOSADA M.; MILEI J.

Milán

Congreso; 25th European Meeting on Hypertension and Cardiovascular Protection; 2015

European Society of Hypertension. International Society of Hypertension

Objective. To evaluate whether usual antihypertensive drugs modify cardiac morphology in spontaneously hypertensive rats (SHR). We show that these drugs modify cardiac function in SHR in other study presented in this congress.Design and method. Male SHR 8 weeks age were randomly assigned to different antihypertensive oral treatment (n=18/group) for 16 months: losartan 30mg/kg (L), hydralazine 11mg/kg (H), rosuvastatin 10mg/kg (R), carvedilol 20mg/kg (C) (n =16), water (SHRw treatment control). Hypertension control group was comprised of 18 normotensive Wistar Kyoto rats (WKY). Histology: conventional light microscopy. Morphology: Image-Pro Plus 6.0 software. Morphometry: area of 237±50 myocytes (transversal section, µm2), number of myocytes/mm2, number of capillaries/mm2 (N). Average oxygen diffusion distance (myocyte-to-capillary) was calculated (R, µm). Stereology: determination of the number of points by counting (Pp), estimation of volumetric density (Vv) for myocardium, interstitium and coronary capillaries as Vv (%) = [Pp /Ptotal] x 100. Statistics: Kruskal Wallis non parametric test, Dunn test for multiple comparisons (Graph Pad Prism, 5.0). Significance: p < 0.05.Results. Table 1 shows full morphometry data; (n) = number of cases, a p