Long-term soft drink consumption causes enlargement of atherosclerotic lesions in ApoE-/- mice. Eur. Soc. Hypertension. 23rd. European Meeting on Hypertension and Cardiovascular

OTERO-LOSADA M.; RODRIGUEZ-GRANILLO G; MC LOUGHLIN S; MULLER A; OTTAVIANO G; MORIONDO M; CUTRIN JC; MILEI J.

Milano

Congreso; 23rd. European Meeting on Hypertension and Cardiovascular Protection; 2013

Eur. Soc. Hypertension.

The effects of long-term soft drink consumption on general metabolism and atherosclerosis were evaluated in genetic apolipoprotein-E deficiency. Previously we reported metabolic and cardiovascular alterations associated with cola drinking in an eugenic animal model. Methods. ApoE-/- mice were randomized in 3 groups according with free access to: water (W), regular cola (C) or light cola (L). After 8 weeks, 50% of the animals in each group were euthanized (Treatment: W8, C8, L8). The remaining mice (all groups) drank water for another 8 weeks (i.e.: 16 weeks from beginning of the study) and were then euthanized (Washout: W16, C16, L16). Body weight (weekly) and food and drink consumption (twice a week) were measured. Blood was collected for biochemical assays. At autopsy, transverse aortic sinus sections were serially cut and stained for histomorphometry and livers and kidneys were processed for microscopic assessment. Results. Soft drinks treatment increased drinking volumes (C8: 4 fold, p