INVESTIGADORES
OTERO-LOSADA Matilde Estela
congresos y reuniones científicas
Título:
Insulin resistance and relative beta-cell volume in sweet carbonated beverage-induced metabolic syndrome in the rat
Autor/es:
OTERO-LOSADA M.; GONZALEZ J; MULLER A; OTTAVIANO G; AMBROSIO G.; MILEI J.
Lugar:
Athens
Reunión:
Congreso; Eur Soc Hypertension Joint Meeting Internat Soc Hypertension 2014; 2014
Institución organizadora:
Eur Society of Hypertension
Resumen:
Purpose: to evaluate insulin resistance, a key factor in metabolic syndrome pathophysiology, and the relative beta-cell volume after 6 months of respectively sucrose-sweet or sucrose-free beverage drinking and possible reversal of the eventual changes in rats. Materials and methods: 48 male Wistar rats were randomly assigned to 3 groups and were respectively allowed to drink regular cola (C) (sucrose sweetened carbonated drink), light cola (L) (low-calorie artificially sweetened carbonated drink) or tap water (W) ad libitum for 6 months. Determinations were performed at 6 months (?Treatment?) and 12 months (?Wash-out?, at the end of an additional 6 months´ tap water drinking period). Glycemia and insulinemia were measured by conventional enzymatic and immunoassay tests and were used to calculate the HOMA-IR index. At autopsy, pancreatic tissue was processed and immunostained for insulin (monoclonal anti-insulin antibody). The relative beta-cell volume (BCV) was quantified on a software-basis. Data were analyzed by MANOVA followed by the least significant difference (LSD) test. Results: cola drinking modified glycemia (F2,30= 6.15, p<0.006), insulinemia (F2,30= 6.59, p<0.004) and HOMA-IR (F2,30= 8.68, p<0.001) whereas time per se affected insulinemia (F1,30=4.66, p<0.04), HOMA-IR (F1,30=4.43, p<0.04) and BCV (F1,30=5.84, p<0.02). In particular, only regular cola drinking (C6) resulted in hyperglycemia (+15%, p<0.07), hyperinsulinemia (+1.4 fold, p<0.03) and HOMA-IR increase (+1.8 fold, p<0.008) compared to water drinking rats (W6). After 6 months wash-out (12 months after the beginning of the study) sustained hyperinsulinemia (+79%, p<0.005) and HOMA-IR increase (+82%, p<0.004) were found in C12 vs W12 group. Compared to W6, BCV was decreased in W12 (-31%, p<0.05), C12 (-47%, p<0.005) and L12 (-36%, p<0.03) and the groups showed no differences between one another. Conclusions: regular cola drinking led to hyperglycemia, hyperinsulinemia, increased insulin-resistance and did not affect BCV. Differently, aging led to a decrease in BCV. A thorough interpretation of presented evidence should consider the possibility that six months cola drinking may be an insufficient time to modify viability of beta cells and reduce BCV as well