Lipid profile and plasma antioxidant status in sweet carbonated beverage-induced metabolic syndrome in rats

MILEI JOSE; OTERO-LOSADA M; GRANA DANIEL R; MULLER ANGELICA; OTTAVIANO GRACIELA; AMBROSIO GIUSEPPE

Oslo

Congreso; 20th European Meeting on Hypertension; 2010

European Society of Hypertension

Lipid Profile and Plasma Antioxidant Status in Sweet Carbonated Beverage-Induced Metabolic Syndrome in Rats: Pp.34.409 Milei, J; Otero Losada, M; Grana, DR; Müller, A; Ottaviano, G; Ambrosio, G Journal of Hypertension. 28():e572, June 2010. doi: 10.1097/01.hjh.0000379947.09834.e2Oxidative stress is involved in the pathophysiology and cardiovascular complications of metabolic syndrome. Objective: To investigate changes in plasma profile of biomarkers of altered redox mechanisms and lipid metabolism, associated with experimentally-induced metabolic syndrome. Methods: Forty-eight male Wistar rats were allowed to drink ad libitum: 1) regular cola (sucrose-sweetened carbonated drink); 2) diet cola (non-nutritive sweetener containing, low-calorie carbonated drink); 3) tap water (controls). After 6 months, all groups were switched to an additional 6 months of tap water drinking (wash-out). Plasma concentrations of the endogenous antioxidants [alpha]-tocopherol and ubiquinone-10 were measured using RP-HPLC with UV detection. Plasma concentrations of glucose, triglycerides, total cholesterol and HDL-cholesterol were measured by standard enzymatic methods. Determinations were performed at 6 months (end of treatment) and 12 months (end of wash-out). Results: In group 1, 6 months of sucrose-sweetened beverage drinking led to significant weight gain (7 +/- 1%, p < 0.01), hypertension (7.5 +/- 0.5% increase in systolic blood pressure, p < 0.01), hypertriglyceridemia (3-fold, p < 0.01), hyperglycemia (15 +/- 1%, p < 0.05), and low ubiquinone-10 levels (52 +/- 2% decrease, p < 0.05). Light cola-drinking rats showed normoglycemia, but an unexpected tendency to hypertriglyceridemia (2-fold, N.S.) and hypercholesterolemia (29% increase in total cholesterol, N.S.); CoQ10 levels were slightly decreased compared to controls (27%, N.S.), but higher than group 1 (51% increase, N.S). These metabolic alterations reversed after wash-out, except for hypertriglyceridemia and low ubiquinone-10 levels. Interestingly, by this time decreased [alpha]-tocopherol concentrations and hypercholesterolemia were observed in all 3 groups (i.e., unrelated to treatment). Conclusions: Experimentally-induced metabolic syndrome induces oxidative stress-related biochemical changes; some of these alterations may be shared by the natural aging process. (C) 2010 Lippincott Williams & Wilkins, Inc.