INVESTIGADORES
OTERO-LOSADA Matilde Estela
artículos
Título:
Lipid profile and plasma antioxidant status in sweet carbonated beverage-induced metabolic syndrome in rat
Autor/es:
OTERO-LOSADA MATILDE E; GRANA DANIEL R; MULLER ANGELICA; OTTAVIANO GRACIELA; AMBROSIO GIUSEPPE; MILEI JOSE
Revista:
INTERNATIONAL JOURNAL OF CARDIOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 146 p. 106 - 109
ISSN:
0167-5273
Resumen:
ABSTRACT Background Oxidative stress seems to be involved in the pathophysiology and cardiovascular complications of metabolic syndrome. Objective To assess possible biochemical alterations in the plasma profile of biomarkers likely reflecting disturbances in redox mechanisms associated with metabolic syndrome. Research Design And Methods Forty eight male Wistar rats were divided in 3 groups and were allowed to drink regular cola (sucrose sweetened carbonated drink), diet cola (non-nutritive sweetener containing, low-calorie sweet carbonated drink) or tap water respectively ad libitum during 6 months. Plasma levels of á-tocopherol and ubiquinone-10 were measured using RP-HPLC-UV detection. Plasma glucose, triglycerides, total cholesterol and HDL levels were measured by conventional enzymatic methods. Determinations were performed at 6 months (end of treatment period) and 12 months (end of a 6 months tap water drinking wash-out period). Results Six months of sucrose-sweet beverage drinking led to mild weight increase (7±1%, p<0.01), hypertension (7.5±0.5% increase in systolic blood pressure, p<0.01), hypertriglyceridemia (3-fold, p < 0.01), hyperglycemia (15±1%, p < 0.05) and low ubiquinone-10 levels (52±2% decrease, p < 0.05). ). Light cola-drinking rats were undistinguishable from either W or C rats according to CoQ10 levels (27 % N.S. reduction vs W, 51% N.S. increase vs C). Normoglycemia as expected and an unexpected tendency to hypertriglyceridemia (2-fold, N.S.) and hypercholesterolemia (29% increase of total cholesterol, N.S.) were observed in the L group. Six months after wash-out alterations reversed except for sustained hypertriglyceridemia and low ubiquinone-10 levels. Interestingly, by this time low levels of á-tocopherol and hypercholesterolemia were found unrelated to treatment (i.e.: all animals controls included). Conclusions Results suggest that metabolic syndrome and the natural aging process might share common oxidative stress-related biochemical disturbances. Keywords: Metabolic syndrome; Oxidative stress; aging, Coenzyme Q10; Ubiquinone; á-tocopherol; vitamin E, triglycerides, cholesterol