INVESTIGADORES
PONCE DAWSON Silvina Martha
congresos y reuniones científicas
Título:
Extracting Information On Single IP3 Receptors From Ca2+ Puffs.
Autor/es:
DANIEL FRAIMAN; GUILLERMO SOLOVEY; SILVINA PONCE DAWSON
Lugar:
Salt Lake City, UT, USA
Reunión:
Congreso; 50th biophysical society annual meeting (2006); 2006
Institución organizadora:
Biophysical Society
Resumen:
The inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) is a Ca2+ channel which provides a link between events at the plasma membrane and Ca2+ release from intracellular stores. Although there is a large body of experimental work on the IP3R, a comprehensive understanding of its kinetics is still lacking. Confocal microscopy and Ca2+-sensitive dyes provide a window with which IP3R dynamics can be studied in a natural environment. This approach has extensively been pursued in Xenopus oocytes, where a variety of Ca2+ signals have been observed. One of the problems associated with this approach is that IP3R´s in the oocyte appear to be organized in clusters so that, unless [IP3] is very small, Ca2+ release through single IP3R´s cannot be observed. The main motivation of this work is to answer the question of whether it is possible to extract information on single IP3R´s from the observation of signals ("puffs") that involve the collective behavior of several channels in a cluster. To this end, we combine a novel statistical analysis of the experimental data with the development of simple models from which we extract some basic features of single IP3R´s such as the typical timescales that characterize channel inhibition.