INVESTIGADORES
SETTON Clara Patricia
congresos y reuniones científicas
Título:
Imaging cell-magnetic nanoparticle interaction
Autor/es:
PAULA A, SOTO; DIEGO CORAL; VANINA USACH; CLARA PATRICIA SETTON; MARCELA FERNANDEZ VAN RAAP
Lugar:
La Plata
Reunión:
Workshop; Imaging Techniques for Biotechnological and Biomedical Applications Workshop; 2016
Resumen:
Exploring magnetic hyperthermia: ferrofluids, in vitro and in vivo experiments.Diego Fernando Coral1, Paula Andrea Soto2, Viviana Blank2, Pedro Mendoza Zelis1,Gustavo Alberto Pasquevich1, Sergio Gonzalez3, Enrique Mario Spinelli3, Alejandro Luis Veiga3, Francisco Homero Sánchez1, Patricia Clara Setton2, Leonor Roguin2, Marcela Beatriz Fernández van Raap11Instituto de Física La Plata, 2Inst. de Química y Físico Química Biológica A. Paladini, 3Instituto de Electrónica, Control y Procesamiento de Señalese-mail: ganimedesdfc@gmail.comTherapeutic and diagnostic methodologies based in nanotechnology are ofimportance nowadays. These protocols are meant be less invasive, more efficientand displaying minor side effects, being endowed of selectivity. Magnetichyperthermia (MH), a modality that uses radio-frequency (RF) magnetic fields toheat single-domain magnetic nanoparticles (NPs), is becoming a powerfuloncological therapy. Iron oxide NPs are the most biocompatible materialsaccepted as a medical device. MH has reached clinical trial, but there are stillunsolved problems like: dosage; NPs spatial distribution in target tissue andtemperature distribution, monitoring and increase control. Dosage is mainlydetermined by NPs´s efficiency to transduce heat (SAR).This efficiency dependson magnetic relaxation and it is highly influenced by NP size, aggregation andinteraction. It is further modified by restricted mobility and confinement inside tumour environment. We have performed MH experiments in ferrofluids [1,2], in vitro [3] and more recently in vivo mice assays. We have analyzed aqueous dispersion of single NPs and randomly or magnetically organized multicore. We proved that the relevant physical parameters for predicting fluid SAR are mean NP volume, its saturation magnetization and a representative mean activation energy, and that SAR depends on concentration [2,4]. In vitro experiments were used to determine uptake, distribution, cytotoxicity and to develop a new methodology for probing intracellular heating in cells cultures [3]. In vivo constitute a more complex scenario because after intratumoral infiltration irregular NP distribution patterns occurs.Also we will present a new portable device, optimized to generate fields of 100kHz and amplitude from 2 to 15 kA/m, versatile for MH research in materials, cell cultures and mice models.[5].[1] 10.1021/jp311556b[2] 10.1021/acs.langmuir.5b03559[3] 10.1021/acs.jpcc.5b12330[4] 10.1039/c6cp08059f[5] Patent Nº 20160101254