Magneto-targeting of adipose-derived stem cells (AdSC) as a peripheral nerve regeneration strategy

PAULA A, SOTO; VANINA USACH; GONZALO PIÑERO; DIEGO CORAL; MARCELA FERNANDEZ VAN RAAP; CLARA P. SETTON

CABA

Congreso; Reunión Conjunta de Sociedades Biomédicas; 2017

Traumatic injury of peripheral nerves has a high incidence worldwide, reinnervation timing after injury is critical to reach nerve recovery. Adipose-derived stem cells (AdSC) are a promising tool for regenerative therapies.The aims of the present work are to demonstrate that iron oxide magnetic nanoparticles are internalized by AdSC and this do not affect cell morphology and viability in culture. Otherwise In vivo experiments are performed to demonstrate that AdSC internalized with magnetic nanoparticles (AdSC-MNP) arrived at the injured nerve at 7 days post-injury and exert a beneficial effect.AdSC were harvested from 60 days old wistar rats and characterized through immunocytochemistry for multipotent cell marker expression. MNP internalization was evaluated through magnetometry experiments and to evaluate viability of AdSC-NPM we perform MTT assays. MNPs were synthetized by co-precipitation technique and widely characterized by high resolution transmission electron microscopy (HR-TEM), nanosight, electron diffraction and vibrating sample magnetometer (VSM)AdSC result positive for CD90 and CD105 and MTT assays showed MNP do not affect cell viability. Epifluorescence microscopy showed the arrival of transplanted AdSC-MNP exclusively to the injured nerve. Western blot analysis shows an increase on MBP levels 7 days post-injury.Further experiments will determine the effect of AdSC-MNP on other aspects of peripheral nerve regeneration.