Differences in lipid profiles from distinct pluripotent cell populations.

CASALI C; USACH V; LAVALLE L; WEBER K; FERNÁNDEZ TOMÉ MC; SETTON AVRUJ CP

Huerta Grande, Córdoba, Argentina.

Congreso; XXVIII Congreso Anual Sociedad Argentina de Neurociencias (SAN); 2013

Differences in lipid profiles from distinct pluripotent cell populations. Casali, CI, Usach, V, Lavalle, L, Weber, K, Fernández Tomé, MC and Setton-Avruj, PC. We have previously demonstrated the ability of bone marrow mononuclear cells (BMMC) to foster remyelination in the injured sciatic nerve, and we are currently conducting experiments to evaluate the same ability in bone marrow stromal cells (BMSC). With the appropriate stimuli, both BMMC and BMSC can differentiate into Schwann-like cells (SLC). For these reasons, the aim of the present work was to evaluate whether BMMC and BMSC change their protein expression and lipid composition once they differentiate into SLC. BMMC were isolated from tibia and femur bones of adult Wistar rats. BMSC were obtained through BMMC culture. SLC were obtained from BMSC differentiation. Schwann cells (SC) isolated from adult rat sciatic nerve were used as control. Protein expression was evaluated by Western blot and flow cytometry. Lipid composition was determined by thin layer chromatography and later quantification. Results indicate that BMMC and BMSC express pluripotent cells markers (CD34 and CD90) but not SC markers (S100β and MBP). SLC increase the expression of S100β and decrease the expression of CD34 and CD90. When lipid composition was analyzed we found that BMSC have ten times more phospholipid (PL) and cholesterol (Cho) contents than BMMC. In contrast, triacylglycerol content in BMSC decreased by 40 % when compared to BMMC. The increase in PL and Cho contents in BMSC suggests the expansion of cellular membranes, which indicates morphological and, probably, physiological changes in BMSC.