Schwann cell differentiation is affected by the redox state.

SALIS C, DAVIO C, SOTO EF, PASQUINI JM, SETTON-AVRUJ CP.; DAVIO C; EF SOTO; JM PASQUINI; SETTON-AVRUJ CP

Pinamar, Buenos Aires, Argentina

Congreso; 10th Congress de la Panamerican Association for Biochemistry and Molecular Biology. XLI Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. XX Reunión anual de la Sociedad Argentina de Neuroquímica.; 2005

SCHWANN CELLS (SCs)  DIFFERENTIATION IS AFFECTED BY THE REDOX STATESalis, C1. , Davio C2, Soto , E.F.1 , Pasquini, J.M.1 and Setton-Avruj, C.P1.1Dept.of Biol Chem, 2Lab Radioisotopos, School of Pharm and Biochem, UBA, Buenos Aires, Argentina      We have previously described that 70 ng/ml Fe3+  and holoTf were able to promote SCs differentiation in vitro through cAMP release and CREB phosphorylation.The aim of the present study was to evaluate if changes in the intracellular redox state induced by the addition of free Fe3+ or as holoTf is involved in this prodifferentiating effect.The content of intracellular oxidant species was measured using a 5 (or 6)-carboxy-2´7´-dichlorodihydrofluorescein diacetate probe (DCDCDHF), which crosses the membrane, and after oxidation, is converted into a fluorescent compound. DNA content was evaluated. Results are expressed as the ratio DCDCDHF/propidium iodide fluorescence. The effect of a potent antioxidant as N acetyl cysteine on P0 levels, a major protein of peripheral myelin was measured by Western blot.Our results demonstrate that from 1 to 5 minutes there is an increase in the intracellular content of oxidant species, that was not observed at 15 minutes. In coincidence with these results, cAMP liberation was observed at 9 minutes. N acetyl cysteine avoids the prodifferentiating effect of Fe3+ on SCs measured by the levels of P0. Further experiments are neccessary to understand the mechanism underlying in the prodifferentiating effect of Fe3+ and the involvement of the redox state in such an effect.