SETTON Clara Patricia
congresos y reuniones científicas
Presence of á globin mRNA and migration of bone marrow cells after sciatic nerve injury.
SETTON-AVRUJ CP, MUSOLINO PL, SALIS C, ALLÓ M, BIZZOZZERO O, VILLAR MJ, SOTO EF AND PASQUÍN JM.; PL MUSOLINO; SALIS CAROLINA; M ALLÓ; O BIZZOZZERO; MJ VILLAR; EF SOTO; JM PASQUINI
Rosario, Santa Fe, Argentina
Congreso; XLII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular, Rosario, Argentina; 2006
Presence of á globin mRNA and migration of bone marrow cells after sciatic nerve injury. Setton-Avruj, C.P*; Musolino, P.L.#; Salis, C.* ; Alló, M.** ; Bizzozero, O§. ; Villar, M.J # ; Soto, E.F* ; Pasquini, J.M* Dept. Quím. Biol., FFyB, IQUIFIB, UBA-CONICET. Facultad de Ciencias Médicas, Universidad Austral E-mail: firstname.lastname@example.org Wallerian degeneration is a well characterized process in which one of the hallmarks is myelin breakdown. We have previously reported that in the distal stump of ligated sciatic nerves, there is a change in the distribution of the major peripheral myelin proteins: P0 and myelin basic protein (MBP) immunoreactivities. These results agreed with the studies of myelin isolated from the distal stump of injured sciatic nerve, showing a gradual increase in a 14 kDa band. The aim of the present study is to dilucidate the identity of the 14 kDa band and its participation in the degeneration-regeneration process.The 14 kDa band was resolved by a 16% gel in two bands containing a mixture of MBP fragments and peptides with great homology with á- and â-globins.The presence of á globin mRNA was demonstrated by real time PCR in the proximal and distal stump of the ipsilateral sciatic nerve. The presence of CD34+ and ED1+ cells was also demonstrated by western blot in isolated myelin from the ipsilateral nerve as well as by immunohistochemistry in the nerves. Labeled cells isolated from normal adult rat bone marrow which were injected intraortically were found to migrate to the injured area by immunofluorescence. These cells could contribute to the remyelination of the damaged area participating in the removal of myelin debris in the distal stump of the injured sciatic nerve.