P0 AND MYELIN BASIC PROTEIN-LIKE IMMUNOREACTIVITIES FOLLOWING LIGATION OF THE SCIATIC NERVE IN THE RAT

SETTON-AVRUJ CP, AQUINO JB, GOEDELMAN C, SOTO EF, VILLAR MJ

Neurochem Res

Plenum Publishing Corporation

Año: 2002 vol. 27 p. 1293 - 1303

In this work we have analyzed variations in the expression of MBPs and P0 in ligated sciatic nerves of young and adult rats at 3, 7 and 14 days post ligation (PL), by immunohistochemistry and SDS-PAGE electrophoresis of isolated myelin. Protein segregation was seen in the distal stump of ligated nerves with the appearance of immuno-reactive clusters. In adult rats, there was an increase of the immunostained area for MBP from 3 to 7, days followed by a marked decrease at 14 days. P0 immunolabeling gave similar results without, however, a decrease at the longer survival time tested. Young animals showed an acceleration in the process of protein redistribution within ligated nerves, which followed a similar pattern to that of adult animals. Electrophoretical  analysis showed a marked decrease in P0 and MBP at 7 days PL in young rats and 14 days PL in adult rats. The functional significance of protein segregation within myelin in injured nerves deserves further analysis.