SETTON Clara Patricia
HoloTransferrin but not apoTransferrin prevents Schwann cell de-differentiation in culture
SALIS C, GOEDELMANN C, SOTO EF, PASQUINI JM, SETTON-AVRUJ CP
Lugar: Basilea; Año: 2002 vol. 24 p. 214 - 221
Schwann cells (SCs) in culture, without the presence of axons, become de-differentiated, reaching a condition similar to that of their precursor cells. Cytoplasmic accumulation of Transferrin (Tf) in myelinated peripheral nerve has been reported and data in the literature support a role for apoTf (aTf) in myelination in the CNS. In the present report, we used SC cultures to evaluate the capacity of apoTf and holoTf (hTf) to prevent cell de-differentiation promoted by fetal calf serum deprivation. SCs incubated in a serum-free medium showed a decrease in the expression of MBP and P0, markers of mature myelin-forming SCs, together with an increase in the levels of p75NTR and GFAP, markers of immature SCs. Treatment with hTf prevented the decrease in expression of MBP and P0 and the increase in p75NTR. ApoTf was unable to prevent these changes except when iron was added to the cultures. These results suggest a role for hTf in the regulation of myelin formation by Schwann cells.