Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

MOGHARBEL, BASSAM FELIPE; FRANCISCO, JULIO CESAR; IRIODA, ANA CAROLINA; DZIEDZIC, DILCELE SILVA MOREIRA; FERREIRA, PRISCILA ELIAS; DE SOUZA, DAIANY; DE SOUZA, CAROLINA MARIA COSTA OLIVEIRA; NETO, NELSON BERGONSE; GUARITA-SOUZA, LUIZ CESAR; FRANCO, CELIA REGINA CAVICHIOLO; NAKAMURA, CELSO VATARU; KAPLUM, VANESSA; MAZZARINO, LETÍCIA; LEMOS-SENNA, ELENARA; BORSALI, REDOUANE; SOTO, PAULA A.; SETTON-AVRUJ, PATRICIA; ABDELWAHID, ELTYEB; DE CARVALHO, KATHERINE ATHAYDE TEIXEIRA

INTERNATIONAL JOURNAL OF NANOMEDICINE

NLM (Medline)

Lugar: LONDRES; Año: 2018 vol. 13 p. 5823 - 5836

1176-9114

Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.