SYSTEMIC TRANSPLANTATION OF BONE MARROW MONONUCLEAR CELLS PROMOTES AXONAL REGENERATION AND ANALGESIA IN A MODEL OF WALLERIAN DEGENERATION

USACH V; MALET M; LOPEZ MARGARITA; LAVALLE L; PIÑERO G; SACCOLITI MARÍA; CUETO A; BRUMOVSKY P; BRUSCO ALICIA; SETTON-AVRUJ CP

TRANSPLANTATION

LIPPINCOTT WILLIAMS & WILKINS

Lugar: Philadelphia; Año: 2017 vol. 101 p. 1573 - 1586

0041-1337

ABSTRACTBACKGROUND: Reinnervation timing after nerve injury is critical for favorable axonal regeneration, remyelination and clinical improvement. Considering bone marrow mononuclear cells (BMMC) are easily obtained and readily available for transplant, this work analyzed the effect of BMMC systemic administration on nerve repair and pain behavior. METHODS: Adult rats with sciatic nerve crush were immediately and systemically injected BMMC through the caudal artery. Non-treated, sham and naïve rats were also included. Histological, immunohistochemical, biochemical, functional and behavioral analyses were performed in nerves harvested from each group at different survival times.RESULTS: Axons in BMMC-treated rats exhibited a more conserved morphological appearance than those in non-treated rats, as observed at different survival times both in semi-thin sections and ultrastructural analysis. BMMC-treated rats also showed a reduction in major myelin protein immunoreactive clusters 7 and 14 days post injury (DPI), as compared to non-treated rats. Electrophysiological analysis showed BMMC treatment to slightly improve the amplitude of compound muscle action potential (CMAP) starting at 14 DPI. Finally, mechanical withdrawal threshold revealed a full preventive action against transient mechanical hypersensitivity in BMMC-treated rats.CONCLUSIONS: These data demonstrate the efficiency of BMMC, systemically and non-invasively transplanted, in correcting morphological, functional and behavioral alterations resulting from peripheral nerve injury.