DNA Barcoding reveals taxonomic conflicts between Sardinella aurita and S. brasiliensis.

MABRAGAÑA E.; DELPIANI, S. M.; GONZÁLEZ CASTRO, M.; ROSSO, J.J.; BLASINA, G.E.; DÍAZ DE ASTARLOA, J. M.

Adelaide

Congreso; Fourth International Barcode of Life Conference; 2011

As part of a general study on DNA barcoding of Argentine fishes, two clupeids of the genus Sardinella were first caught in Mar Chiquita coastal lagoon (MCH) in December 2008. MCH is a temperate shallow coastal lagoon located in Buenos Aires province, Argentina (37º32’-37º45’S 57º19’-57º26’W). The fish fauna of MCH is composed approximately by 28 species, including three clupeids: Brevortia aurea (Spix and Agassiz), Platanichthys platana (Regan) and Ramnogaster arcuata (Jenyns). Two species of Sardinella occur in the southwest Atlantic: Sardinella aurita Valenciennes and S. brasiliensis (Steindachner), but only the former was occasionally reported on Argentine waters. Morphometric, pair and unpair fins rays, and anterior rakers on lower limbs were recorded. Standard DNA barcoding protocols were followed for molecular analysis. Sequence divergences were calculated using K2P. Unrooted NJ tree of available sequences of species of Sardinella in BOLD (Barcode of Life Data System) were build using MEGA 4.1. For “not public” sequences the NJ tree were created using BOLD.