INVESTIGADORES
DIAZ DE ASTARLOA Juan Martin
artículos
Título:
Transcriptomic differentiation underlying marinetofreshwater transitions in the South American silversides Odontesthes argentinensis and O. bonariensis (Atheriniformes)
Autor/es:
HUGHES, L.; SOMOZA, G.M. ; NGUYEN, B. ; BERNOT, J.; GONZÁLEZ CASTRO, M.; DÍAZ DE ASTARLOA, J.M.; ORTÍ, G.
Revista:
Ecology and Evolution
Editorial:
John Wiley & Sons
Referencias:
Año: 2017 vol. 7 p. 5258 - 5268
ISSN:
2045-7758
Resumen:
Silverside fishes in the genus Odontesthes have recently and rapidly transitioned from marine to freshwater, crossing an ecological barrier. Two closely related species, marine O. argentinensis and freshwater O. bonariensis, span this ecological divide, and yet show remarkably little genetic divergence using traditional phylogeographic markers. We sequenced gill transcriptomes from wild-caught O. argentinensis and O. bonariensis to look for candidate genes that might be of ecological importance in the adaptation of these fish to freshwater habitats, and further evaluate whether there is plasticity in O. bonariensis in response to salinity changes in the laboratory that resembles O. argentinensis in expression. We find more than three thousand transcripts differentially expressed, with osmoregulatory/ ion transport genes and immune genes showing very different expression patterns across species. Additionally, we characterize the gill bacterial microbiome from our transcriptomic reads. We also identified more than one thousand transcripts with non-synonymous SNPs in the coding sequences, most of which were not differentially expressed. The diversity of functions associated with both the differentially expressed set of transcripts and those with sequence divergence suggest that multiple abiotic and biotic differences in marine and freshwater habitats are driving transcriptomic differences between these species.