CONICET | Buscador de Institutos y Recursos Humanos

ABSTRACT SUBMISSION Submitted abstract (complete) This is an overview of your submitted abstract. No more changes are possible. If you have questions or remarks please contact the abstract secretariat. Subject: EVALUATION OF NOVEL NEGATIVE MODULATORS OF EPITHELIAL CADHERIN EXPRESSION IN OVARIAN CANCER Abstract: Objectives Ovarian cancer is the 4th leading cause of death in women. However, there are no molecular markers for its detection/monitoring. Epithelial cadherin (Ecad) is a key protein in cell-cell adhesion and its diminished expression is accompanied by cadherin switch (Ecad to neural and/or placental cadherins: Ncad, Pcad) and other Epithelial Mesenchymal Transition (EMT) events, such as expression of transcriptional repressors (Twist, Snail) and mesenchymal markers (vimentin). More recently, a decrease of Ecad has been linked to Dysadherin (Dys) expression, a glycoprotein overexpressed in various tumors and related to tumor metastasis. In ovarian cancer, lower levels of Ecad expression have been associated with metastatic spread. Although EMT has been reported in this cancer, current available data is not conclusive. Moreover, Dys expression has yet not been reported. Aim: To evaluate expression of Ecad, EMT markers and Dys in four ovarian cancer cell lines: TOV-112D (primary tumor), SKOV-3, OAW-42 and OV-90 (ascites). Methods mRNA: standard/real-time PCR, protein: Western immunoblotting/immunocytochemistry. Results An inverse correlation was observed when expression levels (mRNA, protein) of Ecad were compared with those of Twist, Ncad, Pcad, Vimentin, and Dys. Contrasting, Snail expression was similar in all cell lines. Conclusions The analysis of EMT markers led to the identification of cell lines with epithelioid (OV-90), mesenchymal (TOV-112D) or mixed (SKOV-3, OAW-42) molecular profiles. This is the first report that describes the expression of Dysadherin (mRNA and protein) in ovarian cancer.

enviar mensaje