154) Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus.

GUEVARA MA; ROMANOWICZ, E.; GARCIA MENENDEZ, S.; LAFUENTE, J.V.; BAIARDI, G.; GARGIULO P.A.

Mendoza

Congreso; XXXVI Reunión Anual de la Sociedad de Biología de Cuyo. Resumen 152. Mendoza.; 2018

Sociedad de Biología de Cuyo.

154) Guevara M, Romanowicz E, Garcia Menendez S, Lafuente JV, Baiardi G, Gargiulo PA. Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus. XXXVI Reunión Anual de la Sociedad de Biología de Cuyo. Resumen 152. Mendoza. 6 a 7 de Diciembre de 2018.- https://sbcuyo.org.ar/libro-de-resumenes/ACTION OF KETAMINE ON ACQUISITION IN THE NOVEL OBJECT RECOGNITION TEST AND THE METABOLIC ACTIVITY IN HIPPOCAMPUS Guevara M1, Romanowicz E1, Garcia Menendez S1, Lafuente JV2, Baiardi G3, Gargiulo PA1. 1. Laboratory of Neurosciences and Experimental Psychology. Area of Pharmacology. Faculty of Medical Sciences. National University of Cuyo. CONICET. Mendoza. Argentina. 2. Laboratory of Clinical and Experimental Neurosciences (LaNCE). Department of Neuroscience. University of the Basque Country. Leioa. Spain. 3. Laboratory of Neuropharmacology, Institute of Biological and Technological Research (IIBYT-CONICET), National University of Córdoba, Faculty of Chemical Sciences, Catholic University of Córdoba. 5017 Córdoba. Argentina. E-mail: vetguevara@hotmail.com Psychotogenic effects of ketamine, due to antagonism of NMDA (N-methyl-D-aspartic acid) glutamate receptors, are widely known, and they are subject of study in our laboratory. In the present study we searched for the effect of systemic administration of ketamine at low doses in the novel object recognition test. Holtzman derived colony rats weighing 240-290 g were used. Novel object recognition test involves variables related to memory and perception. We used an intraperitoneal administration of ketamine in sub-anesthetic doses (1.25, 2.5 and 5 mg / kg ip) 3 min before training. Test was realized 120 min after training. Additionally, groups of five rats treated with saline or different sub-anesthetic doses were paired for measurement of hippocampus metabolic activity 8 min after injection. It was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through spectrophotometric wavelength measurement. We observed that treatment produced a significant increase in total scan time in the evaluation session (p