147) Effect of Ketamine on anxiety in the plus maze test and the metabolic activity in the amygdala.

GUEVARA, M.A.; GARCIA MENENDEZ, S.; MARQUEZ HERRERO, S.; ROMANOWICZ, E.; BAIARDI, G.; GARGIULO, P.A.

Merlo. San Luis.

Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo. Merlo, San Luis. 6-7 de Diciembre de 2017.-; 2017

Sociedad de Biología de Cuyo.

147) Guevara M, Garcia Menendez S, Marquez Herrero S, Romanowicz E, Baiardi G, Gargiulo PA. Effect of Ketamine on anxiety kn the plus maze test and the metabolic activity in the amygdala. XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo. Merlo, San Luis. 6-7 de Diciembre de 2017. A Publicar en Revista Biocell.- Psychotogenic effects of ketamine, due to antagonism of NMDA (N-methyl-D-aspartic acid) glutamate receptors, are widely known, and they are a subject of study in our laboratory. In the present study we searched for the effect of systemic administration of ketamine at low doses in the plus maze test in Holtzman derived colony rats. Classical anxiety parameters were considered (time spent in the open arm, open and closed arm entries, time per entry and grooming). Three systemically administrated doses were compared with saline vehicle: 1.25, 2.50 and 5 mg/kg (n=20). Five rats of each group were selected for measurement of amygdala metabolic activity. It was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through spectrophotometric wavelength measurement. Statistically significant reduction was observed in the time spent in the open arm and time per entry (p