140) Sedative effect of Gamma-Amino-Butiric Acid in the open field test in rats.

GARGIULO API, ; GUEVARA MA, ; ROMANOWICZ E, ; LANDA, A.I..; MESONES, H.L. ; GARGIULO, P.A.

Mendoza.-

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2016

Sociedad de Biología de Cuyo.-

140) SEDATIVE EFFECT OF GAMMA-AMINO-BUTYRIC ACID IN THE OPEN FIELDTEST IN RATS.Gargiulo API*, Guevara MA*, Romanowicz E, Landa*, Mesones HL*?, Gargiulo PA*?.* Laboratory of Neurosciences and Experimental Psychology. Area of Pharmacology. Faculty of Medical Sciences. National University of Cuyo.(CONICET). ? Argentine Association of Psychiatrists (AAP). E-mail: pagargiulo@hotmail.comGamma-Amino-Butyric Acid (GABA) is a natural compound that mediates an important inhibitory effect within the Central Nervous System (CNS). It is peripherally administered in clinical medicine, but it has been proposed that it may not pass the Blood Brain Barrier (BBB). The aim of the present study is to elucidate the possibility of a central effect when administered peripherally. Male rats weighing 240-270 g from a Holtzman derived colony were used, divided in four groups. These rats were injected with saline or GABA (12.5 and 25 mg/kg) 5 min before testing. The test was the Open Field (Columbus Instruments) in the conditions of our laboratory. Classical parameters were considered: verticalmovements, ambulatory movements, non ambulatory movements, and total number of movements. A significant decrease in ambulatory movements was observed with the middle dose of GABA (p