INCREASED EGR1 EXPRESSION IN THE MEDIAL PREFRONTAL CORTEX AND ALTERED MOTOR AND BEHAVIOURAL PARAMETERS IN THE OFFSPRING FOL- LOWING MODERATE PATERNAL ALCOHOL INTAKE

BIROLO SC; STINSON MG; FESSER E; GOTFRYD L; CAMBIASSO MY; ERLEJMAN AG; CANEPA E; CALVO JC; FONTANA VA

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

Sociedad Argentina de Investigación Clínica

Previously, we observed that male alcohol intake affects spermbiochemical parameters and DNA integrity. The resulting progeny was underweight during their first weeks of life, and overweight in their adulthood, and showed altered spleen cell populations. Aim: To assess the effects of moderate paternal alcohol consumption on different physical and behavioral parameters of its offspring (F1) de- velopment. Additionally, we screened for potential modifications in the expression of candidate genes in the medial prefrontal cortex (mPFC) of F1 mice. CF1 male mice were exposed (A) or not (C) to15% (v/v) ethanol in drinking water ad libitum for 15 days, following which they were mated (1:1) with non-treated CF1 females. Two to17 days old F1 mice were subjected to developmental tests (surface righting, grasping reflex, negative geotaxis, limbs suspension, grip strength, cliff aversion, ambulation tests), and two to four months old F1 mice to behavioral tests (open field or OF, elevated plus maze, object context recognition test and social dominance tube test). Brains from adult F1 mice were dissected, and those from A weighed less than those from C (p