Mouse paternal alcohol exposure affects spermatozoa and compromises the survival of its offspring

GOTFRYD L; SALAMONE G; CAMBIASSO MY; NAGUILA Z; STINSON MG; ERLEJMAN AG; CALVO JC; FONTANA VA

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clìnica; 2018

Sociedad Argentina de Investigación Clínica (SAIC)

Previously we observed that male alcohol consumption delayed embryodifferentiation by deregulation of peri-implantation events and alteration of embryo morphogenesis in vitro. Aim: To evaluate the effect of paternal alcoholconsumption on spermatozoa, embryo and litter survival on a mouse model. Methods: CF-1 male mice were exposed (treated group, T) or not (control group, C) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. Spermatozoa from cauda were obtained by swim-out to determine sperm concentration,motility, head decondensation (15, 30 and 60 min) and apoptosis by AnnexinV-FITC on both groups. Control and treated males were mated with non-treatedCF-1 females (1:1). Pregnant females (positive vaginal plug: day1) were sacrificed at day 2 of gestation to obtain 2-cell embryos which were cultured in vitro for 7 days and apoptosis was determined. Pregnancy outcomes were also evaluated and litter mortality, weight and size registered. Results: Malealcohol consumption did not alter the number of mated female but significantlyincreased sperm head decondensation compared to controls (p