PATERNAL MODERATE ALCOHOL CONSUMPTION MODIFIES MOUSE PERI-IMPLANTATION EMBRYO DEVELOPMENT IMPAIRING INNER CELL MASS AND TROPHOBLAST GROWTH AND MORPHOLOGY

GOTFRYD L; FUENTES F; SANCHEZ MC; SALAMONE G; CALVO JC; CEBRAL E; FONTANA VA

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS. LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC).; 2017

Sociedad Argentina de Investigación Clínica (SAIC)

Previously, we showed that paternal alcohol intake partially inhibits sperm capacitation, increases nuclear decondensation rate and deregulates the dynamics of pronucleus formation and fertilization. Aim: To evaluate the effect of paternal alcohol consumption and its consequences on mouse early embryo development focusing on trophoblast (TB) differentiation and inner cell mass (ICM) as critical embryo stages invading maternal decidua. Methods: CF-1fertile males were exposed (treated group, T) or not (control group, C) to 15%(v/v) ethanol in drinking water ad libitum for 15 days. They were mated with afertile nontreated CF-1 female (1:1). Positive mating females were sacrificedat day 2 of gestation to obtain 2 cell embryos which, then, were cultured for 7 days. Embryo differentiation, growth and morphology were evaluated during pre- and peri-implantation phases in vitro. Then, embryos were classified as type A(ICM: protruding aggregates of compact cells; TB: symmetric monolayer of flat and elongated cells) or type B (ICM: disaggregated, few scattered or no cells,TB: asymmetric trophoblast outgrowth). Frequency differences between C and T were tested by Fisher´s exact test. Results: Male alcohol consumption for 15 days delayed the embryo differentiation and growth by detention/fragmentation and altered the embryo morphology. Differences between type A and B distributionin C and T were statistically significant for ICM and TB in both groups. For ICM: Type A 53% C vs 10% T (p