Identification of soluble components in human adipose tissue explants from normal and tumoral breasts.

FLETCHER SJ; HAPON MB; CALLEGARI E; SERRA MF; LOPEZ-FONTANA CM; SASSO CV; SANTIANO FE; CALVO JC; CARÓN RW; PISTONE CREYDT V

San Luis

Congreso; XXXII Reunión Anual de la Sociedad de Biología de Cuyo; 2014

Among other factors, tumor progression depends on the tumoral epithelial-stromal cells interaction. In the breast, adipose tissue is the predominant stromal type. We have recently demonstrated that conditioned media (CMs) from human adipose tissue explants of tumoral breasts (hATT) regulate proliferation, adhesion and migration of breast cancer epithelial cells. In the present work, we have begun to identify soluble components present in CMs by proteomics. We separated in polyacrylamide gels proteins that were present in both CMs.We lyophilized aliquots from CMs and, by means of two-dimensional nano-liquid chromatography (2D-nanoLC)-mass spectrometry (MS/MS), we began to identify proteins from complex samples. Finally, we analyzed the obtained data with ProteoIQ (Premier Biosoft) software. Compared to CMs-hATN, CMs-hATT had a greater diversity of proteins (at equal total protein) and a higher protein quantity (at equal final volume) (p