Human Periprostatic Adipose Tissue: Secretome from Patients With Prostate Cancer or Benign Prostate Hyperplasia

SACCA PA; MAZZA ON; SCORTICATI C; VITAGLIANO G; CASAS G; CALVO JC

Cancer Genomics & Proteomics

Stanford University HighWire Press

Año: 2019 vol. 16 p. 29 - 58

1109-6535

Abstract. Background/Aim: Periprostatic adipose tissue(PPAT) directs tumour behaviour. Microenvironment secretomeprovides information related to its biology. This study wasperformed to identify secreted proteins by PPAT, from bothprostate cancer and benign prostate hyperplasia (BPH)patients. Patients and Methods: Liquid chromatography-massspectrometry-based proteomic analysis was performed inPPAT-conditioned media (CM) from patients with prostatecancer (CMs-T) (stage T3: CM-T3, stage T2: CM-T2) orbenign disease (CM-BPH). Results: The highest number anddiversity of proteins was identified in CM-T3. Locomotion wasthe biological process mainly associated to CMs-T andreproduction to CM-T3. Immune responses were enriched inCMs-T. Extracellular matrix and structural proteins wereassociated to CMs-T. CM-T3 was enriched in proteins withcatalytic activity and CM-T2 in proteins with defense/immunityactivity. Metabolism and energy pathways were enriched inCM-T3 and those with immune system functions in CMs-T.Transport proteins were enriched in CM-T2 and CM-BPH.Conclusion: Proteins and pathways reported in this study couldbe useful to distinguish stages of disease and may becometargets for novel therapies.