Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake

SÁNCHEZ, MELISA C; FONTANA, VANINA A; GALOTTO, CAMILA; CAMBIASSO, MAITE Y; SOBARZO, CRISTIAN M A; CALVO, LUCRECIA; CALVO, JUAN C; CEBRAL, ELISA

REPRODUCTION

BIOSCIENTIFICA LTD

Año: 2018 vol. 155 p. 529 - 541

1470-1626

Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150min of capacitation in treated males compared to controls (H: 14.1±2.5 vs 23.7±2.6, P