INVESTIGADORES
CHULZE Sofia Noemi
congresos y reuniones científicas
Título:
bacillus velezensis RC 218 as biocontrol agnet of Fuarium head blight : effect on Fusarium gramieanrum penetration and growth on wehat spike
Autor/es:
CANTORO R; PALAZZINI J.M; CHULZE , S.N
Lugar:
Tulln
Reunión:
Congreso; 14 European Fusarium Seminar; 2018
Institución organizadora:
International Society for Mycotoxicology
Resumen:
Fusarium headblight (FHB) causes important yield and quality losses on wheat worldwide. Fusarium graminearum sensu estrictois the main pathogen in Argentina and the disease occurs when hight humidityconditions prevail during anthesis. Among management strategies to control thedisease, biocontrol offers an environmentaly friendly tool to be used in theframe of an  integrated pest management(IPM).. In previous studies we have demostrated the effectiveness of B. velezensisRC218  to reduce both FHB severity andDON accumulation.The aim of the present study was:- to evaluate theeffect of B. velezensis RC218 on F.graminearum spike infection, penetration and deoxynivalenol (DON)production. For this purpose, wheat plants were grown under  greenhouse conditions and inoculated by spray atanthesis stage (DC65) with: i) B. velezensisRC218 and F. graminearum TRI5prom::GFP conidialsuspension (this strain produces a completely functional TRI5 protein,synthetizes GFP protein under the control of TRI5 gene promoter and showsconstitutive expression of dsRed gene)and ii) F. graminearum TRI5prom::GFP. Spikes were sampled at 14days post inoculation and freehand cuts were carried out in different symptomaticspike portions (grain, upper and lower rachis internodes and spikelet glumes).GFP and dsRed production was characterized through FV1000 Olympus confocal microscopeand images were captured. Results showed that B. velezensis RC218 reduced F.graminearum hyphae growth and DON accumulation was more evident in thegrains and its corresponding upper and lower rachis internode. The effect wasnot observed in spikelet glumes. According to these results, B. velezensis RC218 effectiveness overthe pathogen seems to be higher in the spike inner structures, preventingpathogen growth and movement through grains and into the rachis.