Zearalenone, α and β zearalenol levels during ensiling process in a laboratory maize silage model.Zearalenone-binding /biotransorming ability of Saccharomyces cerevisiae strains isolated fronm silage

GONZALEZ PEREYRA, M.L.; SULYOK, M; BARALLA V; CHULZE , S.N; KRSKA R; CAVAGLIERI, L.R

Martina Franca

Congreso; ISM MycoRed International Conference Europe 2013. Global Mycotoxin Reduction Strategies; 2013

ISM

Silage is a major feed source for cattle in Argentina. Fusarium mycotoxins can contaminate maize before ensiling causing reproductive problems in animals. The aims of the present study were: - to determine the effect of the process and the indigenous microbiota on the levels of zearalenone (ZEA), α- zearalenol (α- ZOL) and β- zearalenol (β-ZOL) in artificially contaminated maize, ? to test two Saccharomyces cerevisiae strains isolated from maize silage for their ability to bind/biotransform ZEA in vitro. Twelve polystyrene bags were filled with previously contaminated chopped whole-plant maize and stored in a dry and cool room and sampled at days 7, 45, 90, 120 and 127. S. cerevisiae RC008 and RC009 were incubated shaking in PBS solution additioned with 299; 475.6 or 1,251 ng/ml ZEA for 1h at 25°C. Zearalenone, α- ZOL and β-ZOL levels were quantified by LC-MS/MS. Chemical and physical analysis indicated silage maintained good quality in all stages. pH was reduced favourably (P