INVESTIGADORES
CHULZE Sofia Noemi
congresos y reuniones científicas
Título:
METHOD FOR ALTERNARIA TOXINS ANALYSIS ON WHEAT
Autor/es:
OVIEDO M.S; RAMIREZ M L; CHULZE S.N.
Lugar:
Rosario
Reunión:
Conferencia; 1 Conferencia Latinoamericana ICC 2007; 2007
Institución organizadora:
ICC, INTA, SAGPyA y Bolsa de Comercio de Rosario
Resumen:
Wheat production in Argentina reachs about 16 million tons per year. We have demonstrated that Alternaria is one of the most importat genus present on wheat during non epidemic Fusarium head blight year in Cordoba province, Argentina. We identified only two species: A. alternata and A. infectoria among the Alternaria species isolated. Alternaria alternata strains were able to produced altenuene (ALT), alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TA) and altertoxin I (ATX-I). Among the A. infectoria strains different profiles were found: ALT producers, ALT, AOH and AME producers or AOH producers and no toxin producers. The results suggest a potencial risk to found Alternaria mycotoxins in wheat in Argentina. The aim of the present study was: - to evaluate a method previously described by Solfrizzo et al.(2004) for AOH, AME and TeA determination in wheat. Toxins were extracted from wheat with an acidified mixture of water:methanol:acetonitrile. The filtered extract was divided in two parts that were purified by solid phase extraction on C18 column for the analysis of AOH and AME and on a polymeric Oasis® HLB for TeA, respectively. Toxins were quantified by reverse phase liquid chromatography with ultraviolet detector by using two consecutive isocratic mixtures of acetonitrile:sodium dihydrogen phosphate solution. Mean recoveries of AOH, AME and TeA from wheat spiked at levels between 0.5 and 3 µg/g were 73%, 72% y 70%. Limits of detection (signal-to-noise ratio of 3:1) for AOH, AME and TeA were 0.02, 0.02 and 0.05, respectively.