Analysis of T-2 and HT2 toxins in soybean and soy meal using immunoaffinity clean up and high performance liquid chromatography

BARROS G.G.; GARCIA DAIANA.; OVIEDO M.S; RAMIREZ M L; TORRES A. M.; CHULZE , S.N

Merida

Congreso; VI Latina American Congress of Mycotoxicology and II International Symposium on algal and fungal toxins for industry; 2010

Sociedad Latinoamericana de Micotoxicologia

Soybean (Glycine max L. Merr.) is the main source of protein worldwide used both as food and feedstuffs. Hygienic safety of soybean and by-products depends on fungal contamination among other microorganisms. Fusarium rot of soybeans is described in the literature and different Fusarium species have been isolated from this commodity (Pitt & Hocking 1999). Fusarium species are known to produce a broad spectrum of toxins including type A- and B- trichothecenes. Among A-type trichothecenes, T-2 and HT-2 toxins are relevant mycotoxins produced mainly by F. sporotrichioides, F. poae, F. equiseti and F. acuminatum (Leslie and Summerell, 2006). In a previous study Fusarium species potential mycotoxins producers have been isolated from soybean in Argentina, however limited information about the occurrence of Fusarium mycotoxins in soybean and by-products is available. Aim: to evaluate an HPLC analysis coupled with immunoaffinity column clean-up previously described by Visconti et al. (2005) for simultaneous determination of T-2 and HT-2 in cereal  grains to be applied in soybean and soy meal samples.