Deoxynivalenol and nivalenol analysis in soybean and soy flour

BARROS G.G.; GARCIA D.; OVIEDO M.S; RAMIREZ M.L.; TORRES A. M.; CHULZE S.N.

World Mycotoxin Journal

Wageningen Academic Publishers

Lugar: Wageningen; Año: 2008 vol. 1 p. 263 - 266

1875-0710

  A rapid and accurate method to quantify deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solid-phase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of recovery test with spiked soybean and soy flour samples. The detection limit (LOD) was 0.1 9g/g for DON and 0.2 9g/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit. Quantification limit (LOQ) was established as three times the detection limit. Quantification limit (LOQ) was established as three times the detection limit. Quantification limit (LOQ) was established as three times the detection limit. Quantification limit (LOQ) was established as three times the detection limit. Quantification limit (LOQ) was established as three times the detection limit. 9g/g for DON and 0.2 9g/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit.