Impact of osmotic and matric water stress on germination growth mycelial water potentials and endogenous accumulation of sugars and sugar alcohols on Fusarium graminearum

RAMIREZ, M.L; CHULZE, S; MAGAN, N

MYCOLOGIA.

The Mycological Society of America

Año: 2004 vol. 96 p. 470 - 478

0027-5514

Studies were conducted to determine the effect of osmotic (NaCl, glycerol) and matric (PEG 8000) water stress on temporal germination and growth of two F. graminearum strains over the water potential range of 20.7 to 214.0 MPa at 15 and 25 C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between potential range of 20.7 to 214.0 MPa at 15 and 25 C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between potential range of 20.7 to 214.0 MPa at 15 and 25 C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h incubation. At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between At lower osmotic and matric potentials (27.0 to 28.4 MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between MPa), there was a lag of up to 24 h before germination. Optimum germ-tube extension occurred between C. The effect on endogenous water potentials and accumulation of sugars and sugar alcohols also were measured. For both strains, germination occurred rapidly over the same range of osmotic or matric potential of 20.7 to 25.6 MPa after 4–6 h i