KORNBLIHTT Alberto Rodolfo
A methyl transferase links the circadian clock to the regulation of transcription and alternative splicing
SÁNCHEZ, S. E.; PETRILLO, E.; BECKWITH, E. J.; ZHANG, X.; MATÍAS, L.; RUGNONE, C.; HERNANDO, E.; CUEVAS, J. C.; GODOY HERZ, M. A.; DE PETRIS-CHAUVIN, A.; SIMPSON, C. G.; BROWN, J. W. S.; CERDÁN, P. D.; BOREVITZ, J. O.; MAS, P.; CERIANI, M. F.; KORNBLIHTT, A. R.; YANOVSKY, M. J.
NATURE PUBLISHING GROUP
Lugar: Londres; Año: 2010 vol. 468 p. 112 - 112
Circadian rhythms allow organisms to time biological processes to the most appropriate phases of the day?night cycle1. Post- transcriptional regulation is emerging as an important component of circadian networks2?6, but the molecular mechanisms linking the circadian clock to the control of RNA processing are largely unknown. Here we show that PROTEIN ARGININE METHYL TRANSFERASE 5 (PRMT5), which transfers methyl groups to argi- nine residues present in histones7 and Sm spliceosomal proteins8,9, links the circadian clock to the control of alternative splicing in plants. Mutations in PRMT5 impair several circadian rhythms in Arabidopsis thaliana and this phenotype is caused, at least in part, by a strong alteration in alternative splicing of the core-clock gene PSEUDO RESPONSE REGULATOR 9 (PRR9). Furthermore, genome-wide studies show that PRMT5 contributes to the regu- lation of many pre-messenger-RNA splicing events, probably by modulating 59-splice-site recognition. PRMT5 expression shows daily and circadian oscillations, and this contributes to the medi- ation of the circadian regulation of expression and alternative splicing of a subset of genes. Circadian rhythms in locomotor activity are also disrupted in dart5-1, a mutant affected in the Drosophila melanogaster PRMT5 homologue, and this is associated with alterations in splicing of the core-clock gene period and several clock-associated genes. Our results demonstrate a key role for PRMT5 in the regulation of alternative splicing and indicate that the interplay between the circadian clock and the regulation of alternative splicing by PRMT5 constitutes a common mechanism that helps organisms to synchronize physiological processes with daily changes in environmental conditions.