INVESTIGADORES
KORNBLIHTT Alberto Rodolfo
artículos
Título:
Manganese ion dependent adenylate cyclase activity in rat testes: purification and properties.
Autor/es:
KORNBLIHTT A. R; FLAWIƁ, M. M; TORRES H N
Revista:
BIOCHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 1981 p. 1262 - 1262
ISSN:
0006-2960
Resumen:
Testicular, soluble adenylate cyclase has been purified by anion-exchange chromatography, gel filtration, and isoelectric focusing. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peak fractions from the latter purification step showed only one polypeptide band with an apparent molecular weight of about 69 000. The following hydrodynamic and molecular parameters have been established for this enzyme: sedimentation constant, 4.3; Stokes radius, 3.95 nm; partial specific volume, 0.74 mL.g(-1); molecular weight, 74 000; fractional ratio, 1.4.
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