INVESTIGADORES
VAZQUEZ Elba Susana
congresos y reuniones científicas
Título:
HO-1 INDUCES MX1 EXPRESSION TILTING THE BALANCE OF ENDOPLASMIC RETICULUM STRESS TOWARDS PRO-DEATH EVENTS IN PROSTATE CANCER."
Autor/es:
ORTIZ EMILIANO GERMAN; SANCHIS, PABLO A; BIZZOTO, JUAN; COTIGNOLA JAVIER; VAZQUEZ, E; GUERON G
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual de la Sociedad Argentina de Investigación Clínica 2019; 2019
Resumen:
The principal goal currently within prostate cancer (PCa) research is to unveilmarkers for early detection of aggressive tumors that are predestined to invadeand metastasize. Recent studies suggest that Human Myxovirus Resistant ProteinA (MxA) encoded by MX1 is associated with several human cancers and may be atumor suppressor. In previous bioinformatics study we identified the MX1 as a mostconsistently down regulated gene in PCa vs Normal prostate and Kaplan-Meieranalysis also showed in PCa patients that MX1 loss was associated withdecreased overall and disease-free survival (P≤ 0.05). The previous analysis byRT-qPCR and Immunofluorescence in PC3 cell lines shown an expressionincrement of this possible tumor suppressor in cells with high levels of Heme-oxygenase 1(HO-1)Then, considering that HO-1 is inducible by inflammatory andstress conditions and that is anchored to the Endoplasmic Reticulum (ER), weanalized the expression levels of MX1 in response of this conditions, speciallyunder Endoplasmic Reticulim Stress (ERS) and we assess its influence overApoptosis and Autophagy associated to ERS.Our approach showed an increment of MX1 gene when we induced ERS bythapsigargin in PC3 cells. Furthermore, this increment was associated with rise incellular apoptosis and auophagy in PCa.Therefore, we propose that MX1 tilts the balance of ERS towards pro-death eventsrather than pro-survival according to the possible suppressor role in PCa.